Animal Tissue Bank of Catalunya     Phone +34 93 581 12 35
Departament de Medicina i Cirugía Animal. Facultat de Veterinaria
Universitat Autónoma de Barcelona 08193 Bellaterra  (Barcelona)



 BTAC

About the BTAC
Organization and Responsibles
BTAC Operation
Origin
Processing amd Conservation
Specialized Diagnosis
Data Registry
Quality Control
Consulting Archive
Tissue Request
Requests Evaluation
Applicant's commitments
Material and Costs Procedure
Donations
Ethical Aspects
Biosafety
Interesting Links

Quality is a concept that comes from the industrial and business sector. A tissue bank offers a product, tissues, and it has a plurality of customers, the researchers. Its objective must be thus, to provide the best quality. This means to use a raw material with defined characteristics, stable production, process and a uniform final product, that can be obtained setting up defined working protocols and performing quality controls at the end of the process.

Each manipulation step is controlled in order to minimize the probability that the final product does not accomplish with the required specifications. The quality systems implementation consists in the application of a working methodology to prevent the errors that can be produced during all the process, since the tissue extraction until its sending. This strategy assume more guarantees compared to the tradicional quality control only pointed towards the product.

The objective of the application of the quality systems must be pointed towards a set of critical points or areas that will influence on the final quality and that we can optimize technically. In our case, the special areas of attention are the following:

 1. Specie, individual and tissue selection: criterions, history, controls, etc.
 2. Extraction: Post-mortem time, protocols, packing, etc.
 3. Transport: conditions, temperature, etc.
 4. Register and reception : identification, conservation period, etc.
 5. Manipulation and processing: fixation, freezing, reactives, techniques, devices, etc.
 6. Validation, selection or elimination of pieces, anatomopathological diagnosis, etc.
 7. Register, labelling, archive, etc.
 8. Storage: temperature, conditions, caducity, etc.

 9. Distribution: transport conditions, documentation, standard usage, etc.
 10. Benefits pursuit coming from its usage and the research results.

The BTAC also performs a quality control of its products (tisssue samples) in order to guarantee that the stored material is suitable for research. The quality control established in this first step consists in:

• Microscopical valuation of the histological sections: representative sections are obtained, histologically stained and analyzed in the microscope in order to evaluate the structural integrity of the tissue.

The BTAC has the project of increasing its sample quality control through:

• Molecular integrity evaluation: a representative sample of fresh tissue is taken and frozen, the RNA (molecule specially sensible to degradation) is extracted and its integrity is evaluated.

• Evaluation of gene expression associated with cellular/ tissue hypoxia: study at mRNA and/or proteins level .

MIC3